摘要
目的 探讨细菌脂多糖（LPS）对粉螨变应性鼻炎患者细胞因子分泌的影响.方法 选择常年性变应性鼻炎患者8例,取同一患者三份肝素抗凝静脉血,加入胎牛血清最终体积为1 ml,两个实验组分别加入10 μg或1μgLPS,即高、低LPS剂量组,对照组不加.酶联免疫吸附测定（ELISA）法检测IL-4、IL-5、IL-10、IL-12、IFN-γ含量.结果 发现IL-4、IL-5、IL-10、IL-12生成量有统计学意义,IFN-γ生成量差异无统计学意义.两两比较,两实验组之间及高剂量组与对照组之间的IL-4、IL-10、IL-12的生成量有统计学意义,而低剂量组与对照组间无统计学意义;三组间两两比较,IL-5生成量皆有统计学意义,低剂量LPS对其产生有明显促进作用,而高剂量LPS有显著抑制作用;IFN-γ生成量在各两组间皆无统计学意义.结论 LPS可有效凋节常年性过敏性鼻炎患者的细胞因子分泌及Th1/Th2平衡,并且具有剂量依赖性,提示其在常年性变化性鼻炎患者特定细胞因子靶向性治疗上的应用前景.
Objective To explore the influence of bacteria lipopolysaccharide on the cytokine production of flour mite allergic rhinitis patients. Methods 8 cases with perennial allergic rhinitis were selected. Then the heparin anticoagulation venous blood was taken from the same patient and was added to fetal calf serum,with ultimate volume 1 ml. The two experimental groups were added 10μg LPS and 1 μg LPS respectively, forming the high LPS dose group and low LPS dose group,while the control group was added nothing. Results The contents of IL-4、IL-5、IL-10、IL-12、IFN-γwere tested by enzyme-linked immunosorbent assay. The findings were that there was significant difference in the productive contents of IL-4、IL-5、IL-10、IL-12 and no significant difference in that of IFN-γ between the high LPS dose group and the low LPS dose group. When compared in two groups,significant difference was found in the productive contents of IL-4 ,IL-10 and IL-12 between the two experimental groups and between the high LPS dose group and the control group, while no obvious difference existed between the low LPS group and control group. However, significant difference was altogether found in the productive content of IL-5 when compared one with another among the three groups,and the result was that low dose of LPS obviously promoted the production of cytokine while high dose of LPS restrained the production of cytokine; no significant difference was found in the production of IFN-γ in all comparisons between the three groups. Conclusion This research, through the experiments in vitro, proved that LPS could effectively adjust the cytokine production of perennial flour mite allergic rhinitis patients and the balance of Th1/Th2 ,whose effect depends on the dose of LPS. This research suggested that the practical future in which special cytokine target treatment will be adopted on perennial flour mite allergic rhinitis patients.
出处
《中国基层医药》
CAS
2010年第24期3322-3325,共4页
Chinese Journal of Primary Medicine and Pharmacy
