摘要
目的：对横根费菜的组织形态进行鉴别,并建立横根费菜中没食子酸含量测定方法。方法：采集原植物,对该植物进行植物学及生药学的鉴定;采用HPLC-UV法,色谱柱为Hypersil C18柱（4.6 mm×250 mm,5μm）;流动相：甲醇-体积分数为0.3%磷酸水溶液（94∶6）;检测波长：273 nm;流速：1.0 mL/min。结果：横根费菜的生药学特征明显。根状茎木质,横生有分枝;叶横切面上、下表皮细胞气孔多为不等式、环式,叶脉维管束为周木型;茎维管束外韧型,茎的表皮层和髓较宽阔,导管多为螺纹,可见网纹。没食子酸在0.12~1.20μg质量范围内呈良好线性关系（r=0.999 9）。结论：横根费菜生药学特征明显,易于观察;没食子酸的含量测定方法简单、快捷、准确,为制定横根费菜药材质量标准提供依据。
Objective：To identify the tissue structure of Sedum kamtschaticum Fish,and establish a method for the determination of gallic acid in Sedum kamtschaticum Fish.Methods：The plant was collected to study on the botanical and pharmacognostical characteristics.The determination by HPLC was performed on a hypersil column C18（4.6 mm×250 mm,5 μm） by UV detector at 273 nm with methanol-0.3% phosphoric acid（94：6） as the mobile phase,and the flow rate was 1.0 mL/min.Results： Sedum kamtschaticum Fish was of pharmacognostical characteristics of rhizome lignin,diageotropism with branches;the inequality and concentric type in most of leaf cross section top and bottom epidermal cell blowhole;amphivasal leaf vein bundle;collateral vascular bundle,broad stem＇s cuticular layer and the marrow,thread of plant duct with obvious cobwebbing.The calibration curve showed a good linearity within the range of 0.12~1.20 μg（r=0.999 9）.Conclusion： The pharmacognostical characteristics of Sedum kamtschaticum Fish are obvious.The method is proved to be simple,accurate which provides the basis for controlling the quality of Sedum kamtschaticum Fish.
出处
《河南大学学报（医学版）》
CAS
2011年第1期32-35,共4页
Journal of Henan University:Medical Science
基金
河南大学2009年大学生创新性实践项目(09NA014)
