摘要
为观察葡多酚(GPC)对小鼠乙醇性肝损伤的防护作用,将每天经口灌胃给予4g.(kg.bw)-1乙醇的小鼠同时分别给予不同剂量的GPC,30d后处死小鼠;取肝组织用MTT法检测各组小鼠肝细胞的增殖活性,用免疫组化法和图像分析方法检测PCNA和Bcl-2表达水平。结果显示:GPC高剂量组16h的肝细胞增殖活性为0.423±0.125,较乙醇对照组显著性升高(p<0.05)。GPC高剂量组PCNA增殖指数和Bcl-2阳性表达率分别为39.26%和33.78%,乙醇对照组则分别为9.5%和9.22%。图像分析OD值则高剂量组为0.449±0.041和0.376±0.048,乙醇对照组为0.342±0.040和0.281±0.036,各组间均有显著性差异(p<0.05)。由此可以得到结论:GPC可抑制乙醇诱发的小鼠肝细胞增殖活性损伤及Bcl-2水平降低,对小鼠乙醇性肝损伤有防护作用。
To study the protective effect of grape procyanidins（GPC） on liver injury induced by ethanol in mice,the mice were orally given 4 g·（kg·bw）-1 ethanol with different doses of grape procyanidins once a day.After 30 days,the mice were killed.The proliferation activity of the hepatic cells was determined by MTT assay;the levels of PCNA and Bcl-2 protein expression in the cell were measured using immunohistochemistry method and image analysis method.Results show that the proliferation activity of the high-dose GPC group was 0.423±0.125,significantly higher than that of the ethanol group（p 0.05）.The proliferation index of PCNA protein and the expression rates of Bcl-2 protein of the high-dose GPC group were 39.26% and 33.78%,respectively,and those of the ethanol group were 9.5% and 9.22%,respectively.The OD value of PCNA and Bcl-2 protein of the high-dose GPC group were 0.449±0.041 and 0.376±0.048,respectively,those of the ethanol group were 0.342±0.040 and 0.281±0.036,respectively.There were significant differences between these groups（p 0.05）.It indicated that GPC had inhibitory effect on the decrease of proliferation activity and Bcl-2 protein expression in mice induced by ethanol.It had protective effect on liver injury induced by ethanol in mice.
出处
《生态毒理学报》
CAS
CSCD
2011年第1期105-109,共5页
Asian Journal of Ecotoxicology
基金
青岛市科技发展计划项目(No.072115nsh)
