摘要
目的 探讨染色体微卫星不稳定性（ ＭＳＩ） 及杂合性丢失（ＬＯＨ） 在膀胱癌早期诊断中的意义。 方法 运用位于４ 、５ 、９ 、２１ 号染色体上的四对微卫星标志，结合ＰＣＲ 银染技术，分别检测２２例膀胱癌患者肿瘤组织及尿脱落细胞染色体ＭＳＩ及ＬＯＨ。 结果 肿瘤及尿液标本ＭＳＩ的发生率分别为２３ ％ 、１９ ％ ，ＬＯＨ 发生率分别为４１ ％ 、１８ ％ ，至少有一个位点出现ＭＳＩ或ＬＯＨ 分别为５９ ％ 及３２ ％ 。不同年龄、性别、临床分期、肿瘤组织分级的患者ＭＳＩ与ＬＯＨ 的发生率差异均无显著性（ Ｐ＞０ ．０５） 。 结论 检查尿液中肿瘤脱落细胞的ＭＳＩ及ＬＯＨ 可能成为膀胱癌筛选及监测复发的辅助方法。
Objective To evaluate the loss of heterozygosity (LOH) and microsatellite instability (MSI) in the early diagnosis of bladder cancer. Methods LOH and MSI were studied in the tumor tissue and urine sediment of 22 cases of bladder cancer by means of PCR amplification of 4 polymorphic microsatellites DNA loci on chromosome 4,5,9 and 21. Results 5 tumors (23%) and 4 urine sediments (19%) showed MSI;10 tumors (41%) and 4 urine sediments (18%) showed LOH at one or more loci.Molecular genomic change (MSI or LOH) at one or more loci was observed in 59% of the tumors (13/22) and in 32% of urine sediments (7/22).The frequency of either MSI or LOH was not significantly related to the tumor grade,tumor stage,patients's age or sex ( P >0.05). Conclusions DNA microsatellite analysis of droped tumor cells in urine might be a useful additional method for early diagnosis of bladder cancer.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1999年第10期611-613,共3页
Chinese Journal of Urology
基金
卫生部基金
