摘要
目的 用微卫星标记技术寻找与白血病发生相关的候选抑癌基因。方法 用聚合酶链反应 (PCR)扩增方法检测 2 8例白血病患者 11号染色体短臂四个微卫星位点的杂合性缺失频率。结果 18例急性白血病患者中 ,8例 (44 .4 % )在 11号染色体短臂上出现了至少 1个位点的杂合性缺失 (LOH) ;10例慢性白血病患者中未检出所选位点的LOH。其中位点D11S1334(位于 11p15 .4 p15 .2 )的LOH率为 2 7.8% (5 / 18) ,位点D11S1331(位于 11p15 .4 p 15 .1)的LOH率为 2 2 .2 % (4/ 18)。位点D11S92 2 (位于 11p15 .5 )LOH率为 2 2 .2 % (4/ 18) ,且有 75 % (3/ 4 )的患者同时存在该位点与其它位点的改变。结论 在 11p15 .2 p 15 .
Objective To use microsatellite markers to find and locate the loss of tumor suppressor gene in leukemia. Methods Amplification of 4 microsatellite loci on 11p in 28 cases of leukemia(18 cases of acute leukemia and 10 cases of chronic leukemia) for the loss of heterozygosity(LOH). Results LOH at least one locus was found in 8 of 18 acute leukemia(44.4%), but not in chronic leukemia. Frequency of LOH at locus D11S1334(11p15.4 15.2) and D11S1331(11p15.4 15.1) was 27.8%(5/18) and 22.2%(4/18) respectively. In 4(4/18) cases acute leukemia LOH was observed in D11S922(11p15.5) and in 3 of 4 simultaneous LOH at both this locus and other loci was demon strated. Conclusion Our study indicates that from 11p15.2 to the terminal loci of 11p may harbor a tumor suppressor gene associated with the progression of acute leukemia.
出处
《上海医学检验杂志》
北大核心
2002年第2期93-95,共3页
Shanghai Journal of Medical Laboratory Sciences
基金
山东省卫生厅资助 (970 16)
关键词
微卫星标志
杂合性缺失
抑癌基因
白血病
Microsatellite
Loss of heterozygosity
Tumor suppressor gene
Leukemia
