摘要
目的：探讨尿沉渣ＤＮＡ微卫星分析方法在中国人膀胱癌诊断中的应用价值。方法：首先选取分布于１８条染色体上的 ６０个微卫星位点对 ３８例膀胱肿瘤病人的尿沉渣和相应病理组织的 ＤＮＡ进行微卫星分析，寻找覆盖病人最多的位点组合；然后以此组合对４０例膀胱疾病病人和健康志愿者的尿沉渣进行了双盲分析，以验证此组合的特异性和可能的临床价值。结果：建立了一套由９个微卫星位点组成的诊断组合。 ５９例膀胱癌中， ８６％（３１／３６）的癌组织和９０％（５３／５９）的尿沉渣ＤＮＡ在这９个位点中至少有一个位点发生异常；１２例非膀胱肿瘤个体的尿沉渣ＤＮＡ无一例发生改变；检出率与肿瘤分期、分级无相关性；而且，该组合与美国人报道的组合明显不同。结论：作为一种无创伤性手段，尿沉渣ＤＮＡ微卫星分析对于膀胱癌的筛查和早期诊断具有一定意义；与美国报道的诊断组合的不同，反映了人种差异和可能的病因学差异。
Objectives: To investigate microsatellite analysis of urine sediments and find the potential for using it in diagnosis of bladder cancer in the Chinese. Methods: Sixty microsatellites markers from 18 chromosomes were first tested in a penal of 38 bladder tumors and corresponding urine specimens. The markers with high informative rates and frequent alterations were finally selected to set up the diagnostic panel. The same study was performed on urine DNA from 40 individuals with or without bladder lesions in a blinded fashion to test the specificity and the possible clinical value of this method. Results: A panel consisting of 9 microsatellites for diagnosis was set up. Among the 59 cases of bladder cancer, 86% (31 /36) of tumor tissues DNA and 90% (53/59) of urine sediments DNA showed alterations, at least, in one of the nine microsatellite markers, while the 12 non-bladder-cancer individuals had no changes at any of these loci. The result was not associated with grade or stage of the tumor. Notably, this panel is mostly different from that used for the American patients. Conclusions: Microsatellite analysis of urine is a usable non-invasive method for early detection of bladder cancer in the Chinese. The distinction between this panel and that of American implied racial and possible etiological differences.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第6期512-516,共5页
Chinese Journal of Cancer
基金
国家"九五"攻关项目!( 96-906-01-14)
