摘要
目的 探讨放射免疫显像对人绒癌裸鼠皮下移植瘤的显像效果。方法 应用１３１Ｉ标记的小鼠抗ｈＣＧ单克隆抗体，对人绒癌裸鼠皮下移植瘤模型进行放射免疫显像，以正常小鼠ＩｇＧ 作为对照，观察放射免疫显像效果，并测定不同组织中的放射性活度，计算肿瘤非肿瘤放射活性比（ＴＮＴ比值） 。结果 在注入标记抗体后２４ 小时，移植瘤部位即可见放射性浓聚，并随时间的推移而浓聚增强，７２～９６ 小时后肿瘤可以清晰显像，可显像的最小移植瘤直径为０－８ ｃｍ 。ＴＮＴ比值亦随时间的推移而增高，并明显高于对照组。
Objective To study the efficiency of radioimmunodetection to locate xenograft of human trophoblastic cancer in nude mouse Methods Radioimmunodetection was performed with a cocktail of 131 I labeled mouse anti hCG monoclonal antibodies to image xenogaft of human trophoblastic cancer in nude mice. Normal mouse IgG used to treat mice bearing trophoblastic cancer xenograft was used as control. Radioactivity in different tissues was measured and the tumor/non tumor(T/NT) ratio was calculated Results The accumulation of radioactivity in the xenograft could be recognized as early as 24 hours after the injection of the radiolabeled anti hCG antibodies. Radioactivity accumulation became increasingly evident with time. At 72～96 hours after injection of the radiolabled antibodies, the xenograft could be clearly shown. The minimal size of the xenograft with demonstrable radioactively was 0.8 cm in diameter. The T/NT ratio increased with time and was obviously higher than that in mice treated with normal mouse IgG. Conclusion Radioimmunodetection can efficiently locate human trphoblastic cancer xenograft in nude mice.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1999年第5期342-344,共3页
Chinese Journal of Oncology
基金
国家863 项目基金
关键词
绒毛膜癌
肿瘤移植
疾病模型
放射免疫显像
Choriocarcinoma Neoplasm transplantation Disease models,animal Radioimmunodetection Nude mice
