摘要
评价ELISA法用于筛选猪瘟抗体阴性猪的可行性,分别采用两种商品化猪瘟抗体ELISA检测试剂盒检测了61份猪血清样品,并与兔体中和试验方法进行了比较。兔体中和试验法检测出4份阳性、2份可疑、55份阴性,而两种ELISA试剂盒均检测出6份阳性、55份阴性;两种ELISA方法与兔体中和试验检测结果阴性符合率均为100%(55/55)。结果表明,ELISA法更加敏感,可以替代兔体中和试验方法用于筛选猪瘟抗体阴性猪。
To evaluate the feasibility of using the ELISA method to select classical swine fever antibody-negative pigs,61 serum samples of pigs were detected by two commercial ELISA kits and rabbit neutralization test（RNT）,respectively.The number of negative samples,positive samples and suspicious samples were 55,4,2 detected by RNT,and 55,6,0 by the two ELISA methods,respectively.Comparing the results of three methods,the negative coincidence rate was 100%（55/55）.The result showed that ELISA was more sensitive than RNT,and can replace RNT to select classical swine fever antibody-negative pigs.
出处
《中国兽药杂志》
2011年第1期20-21,共2页
Chinese Journal of Veterinary Drug
基金
国家科技部科技基础性专项工作"重大动物疫病病原及相关制品标准物质研究"项目(2008FY130100)
关键词
猪瘟
ELISA
兔体中和试验
classical swine fever
ELISA
rabbit neutralization test
