摘要
本文介绍了5例ANLL 患者骨髓L-CFU 体外完全无血清培养方法。培养体系中必须加入某些物质,以取代小牛血清。结果表明,加入2×10^(-4)mol/LBSA 第5部份、400μg/ml 饱和铁的人运铁蛋白、1U/ml 胰岛素及20μg/ml 胆固醇,对L-CFU 生长十分重要。加入1×10^(-4)mol/L2-ME 可使L-CFU 集落增大。全部实验均用HTB9-CM 作为刺激因子。本方法为研究人类造血生长因子对L-CFU 增殖调控以及将来L-CFU 培养的标准化,提供了先决条件。.i.———.———.一…..、.
A method using completely serum-free culture system for L-CFU assay in vitro
on 5 cases of acute non-lymphocytic leukemia is reported.Some agents were tested
for the requirement of L-CFU assay in order to replace the bovine serum using in the
conventional culture medium.The result shows that fraction V of bovine serum albu-
min (2×10^(-4)mol/L),iron saturated human transferrin(400μg/ml),insulin (1U/ml),and
cholesterol(20μg/ml)are the basic components for ANLL L-CFU growth.Increase
in colony size can be obtained by adding 2-Mercaptoethanol.HTB9-CM as growth sti-
mulating factor was used in all experiments.This method may be critical for the study
of L-CFU proliferation regulation by human hemopoietic growth factor and the stan-
dardization of L-CFU assay in the future.
出处
《苏州医学院学报》
1990年第1期53-56,85,共4页
Acta Academiae Medicinae Suzhou
关键词
白血病
L-CFU
无血清培养
acute non-lymphocytic leukemia
L-CFU
serum-free culture
