摘要
目的:构建胃低分化腺癌组织cDNA消减文库,筛选胃癌特异表达基因。方法:取胃低分化腺癌组织和癌旁正常黏膜组织,提取总RNA,逆转录cDNA,进行SSH构建消减文库获得序列标签,与载体连接、克隆、筛选、测序及同源性检索。结果:以癌旁正常黏膜组织为driver、胃低分化腺癌组织为tester成功构建cDNA消减文库,测序结果与公共数据库中已知基因和人类EST比较,获得5个特异性序列标签(NBPF1、FAM48A、RXFP2、ACTB和QDPR)。结论:应用SSH法成功构建胃低分化腺癌组织cDNA消减文库,初步筛选胃癌部分表达基因,为寻找川北地区胃癌发生发展的特异性基因奠定基础。
Objective： To construct cDNA subtracted libraries from gastric poorly differentiated adenocarcinoma and screen differentially expressed genes.Methods： Relatively pure gastric poorly differentiated adenocarcinoma and normal gastric mucous membrance were procured,and abstracted the total RNA,and the cDNA was been reversed transcription,which was used to carry on for suppression subtractive hybridization（SSH）.Subtracted cDNA fragments were linked with vector,cloned,screened,sequenced,and made homologous search.Results： The subtracted cDNA libraries were constructed with the tester of gastric poorly differentiated adenocarcinoma and the driver of normal mucosa tissue beside of the dysplasia.The sequenced result compared with the known genes of PubMed and human EST and five specificity sequences were procured（NBPF1,FAM48A,RXFP2,ACTB and QDPR）.Conclusion： Subtracted cDNA libraries from gastric poorly differentiated adenocarcinoma using SSH.Some fragments have been screened and verified to help to search for novel associated genes with gastric cancer of North Sichuan Area.
出处
《中国医药导报》
CAS
2011年第5期19-21,共3页
China Medical Herald
关键词
胃癌
SSH
特异基因
Gastric cancer
Suppression subtractive hybridization
Special gene
