摘要
目的筛选食道癌发生、发展和转移过程中表达变化的基因。方法使用基于抑制性PCR技术的消减杂交方法,对食道鳞状上皮细胞癌组织与癌旁正常黏膜组织cDNA进行消减杂交,并对食道癌组织表达发生变化的基因克隆、测序及同源性分析。结果对等量的食道鳞状上皮细胞癌组织与癌旁正常黏膜组织cDNA进行消减杂交后,获得4个与食道癌发生与转移有关的序列标签,经测序和同源性检索证实为食道癌相关基因2、胱抑素B、膜联蛋白A1、钙粒蛋白A,它们的表达变化可能与食道癌的去分化、恶性增殖转化有关。结论抑制性消减杂交技术可高通量地分析食道癌发生、发展和转移过程中基因表达的变化。
Objective To screen the specific expressed genes of esophageal cancer.Methods The authors prepared the cDNA of esophageal squamous cell cancer and adjacent normal mucosa tissues,and used in differential hybridization by suppression subtractive hybridization based on PCR,the specifically expressed sequence tags cDNA in esophageal cancer tissue were cloned,sequenced and analyzed.Results 4 expressed sequence tags were obtained,including esophageal cancer related gene 2,Cystatin B,Annexin A1,calgranulin A.These tags may involve in esophageal cancer dedifferentiation, proliferation and transformation.Conclusions Suppression subtractive hybridization is an effective method with high specificity and high-flux to detect specific expressed genes in esophageal cancer genesis,development and transferred processes.
出处
《川北医学院学报》
CAS
2007年第5期421-424,共4页
Journal of North Sichuan Medical College
基金
四川省教育厅自然科学重点项目基金资助(编号:2006A059)
关键词
食道癌
SSH
特异表达基因
Esophageal cancer
Suppression subtractive hybridization
Specific expressed genes
