摘要
采用草鱼生长激素夹心式酶联免疫吸附测定法（ＥＬＩＳＡ） ，研究了重组鲤生长激素（ｒｃＧＨ） 在胡子鲶血浆中的代谢动力学。肌肉注射ｒｃＧＨ（０．２ 和ｌ μｇｇ） 后， 胡子鲶血浆ｒｃＧＨ 水平迅速升高， 于２ 和３ｈ 时达峰值， 随后下降，在注射后第２ 和４ 天， 血浆中检测不到ｒｃＧＨ。腹腔注射ｒｃＧＨ（０ ．２ 和ｌμｇｇ） 后， 胡子鲶血浆ｒｃＧＨ水平也有同上类似的变动规律。胡子鲶腹腔注射０ ．５、１ 和２ μｇｇ 的ｒｃＧＨ，７ 天后各组鱼血浆中的ｒｃＧＨ 均被清除干净。向尾部血管内注射ｒｃＧＨ（０ ．２ 和０．５ μｇｇ） 后，胡子鲶血浆ｒｃＧＨ 水平下降遵循指数性衰减规律，２４ｈ 后血浆中ｒｃＧＨ 均未清除干净。
Using the sandwich enzyme linked immunosorbent assay(ELISA) for grass carp growth hormone , plasma kinetics of rcGH in Clarias batrachus after administrations of rcGH were investigated. After intramuscular injections of rcGH (0.2 and l μg/g) into C.batrachus,the plasma rcGH levels were increased, peak values were seen at the end of 2 and 3 hours post injection,and rcGH in plasma was undetected on the 2nd and 4th days post injection, respectively. Similar uptake and clearance patterns were seen after intraperitoneal injections rcGH(0.2 and l μg/g) into C.batrachus . Intraperitoneal injections of rcGH (0.5,1 and 2 μg/g) to walking catfish, rcGH in plasma was all undetected on the 7th day post injection. When rcGH( 0.2 and 0.5 μg/g) was injected into intra caudal vessel of C.batrachus , plasma rcGH levels decreased by way of exponential decay,rcGH in plasma was detectable 24h postintra caudal vessel injection.
出处
《水产学报》
CAS
CSCD
北大核心
1999年第3期234-240,共7页
Journal of Fisheries of China
基金
农业部九五重点科研资助
关键词
胡子鲶
重组鲤生长激素
血浆代谢动力学
Clarias batrachus, Recombinant common carp growth hormone (rcGH), Plasma metabolic kinetics
