摘要
采用草鱼生长激素夹心式酶联免疫吸附测定法和酶联免疫吸附受体测定法 ,研制肠溶微囊式重组鲤生长激素 (rcGH)促生长剂。结果表明 ,肠溶树脂Ⅰ和Ⅱ溶解的pH阈值分别为 6 0和 6 6。当大于阈值时 ,肠溶微囊Ⅰ和Ⅱ内rcGH的释放率随pH值升高和时间延长而逐渐升高 ,直至rcGH完全释放。投喂胡子鲶 (Clariasbatra chus)含肠溶微囊Ⅰ (内含rcGH)后 ,血清rcGH水平 15h后开始升高 ,2 4h时达最大值 ,随后呈下降趋势 ,48h后血清中检测不到rcGH ;而投喂含rcGH的药饵 (1mgrcGH/g饵料 ) ,6～ 6 0h在胡子鲶血清中均未检测到rcGH。说明肠溶微囊Ⅰ对rcGH起一定保护作用 ,肠溶微囊Ⅰ和Ⅱ具有抗酸肠溶胶囊的特点和作用。
Enhancer of growth of microcapsule-type recombinant common carp (Cyprinus carpio) growth hormone (rcGH) was investigated with the sandwich enzyme-linked immunosorbent assay (ELISA) for Chinese grass carp (Ctenopharyngodon idellus) growth hormone and enzyme-linked immunosorbent receptor assay (ELISA-RA) for fish.The pH threshold values of dissolution at ratesin Ⅰ and Ⅱ were 6.0 and 6.6,respectively.At pHs above threshold value,the releasing rate of rcGH from microcapsule Ⅰand Ⅱ increased gradually as pH values increased and time went on.Following feeding of microcapsule Ⅰcontaining rcGH or rcGH (1?mg rcGH/g food pellets) to Clarias batrachus,serum rcGH levels of the former started to elevate after 15?h,reaching a maximum after 24?h,and undetected at the 48?h after administration,whereas rcGH in serum of the latter was undetected from 6 to 60?h.It is quite clear that microcapsuleⅠplays a role in protecting rcGH againt degrading by the proteinase in the digestive tract to some extent and microcapsuleⅠand Ⅱ possess the characteristics and function of antacid capsule.
基金
国家自然科学基金资助项目 ( 395 70 0 99)
农业部"九.五"重点资助项目 [农业部 (渔 ) 96 -0 3]
广东省自然科学基金助项目 ( 980 30 8)
关键词
胡子鲶
重组鲤生长激素
肠溶微囊式生长激素促生长剂
Clarias batrachus
Recombinant common carp growth hormone
Additive of enhancing growth microcapsule-type growth hormone
