摘要
目的:研究3种五环三萜化合物齐墩果酸(OA)、乌苏酸(UA)、积雪草酸(AA)对肝细胞线粒体高通透性转运(MPT)的影响。方法:运用体外高钙诱发MPT模型,测定OA,UA,AA对肝细胞线粒体肿胀、线粒体膜电位耗散、线粒体内游离钙释放的影响。结果:正常小鼠肝细胞线粒体中加入50μmol.L-1 Ca2+即可引起明显的线粒体肿胀、膜电位耗散以及游离钙的释放,而50 mg.L-1 OA,UA,AA均可有效抑制上述过程,且3种化合物的抑制能力存在显著差异。结论:3种五环三萜化合物OA,UA,AA均具有显著对抗MPT,进而保护肝细胞线粒体的功能,且AA的作用强于UA及OA。
Objective： To study effects of three pentacyclic triterpenoids, oleanolic acid （ OA）, ursolic acid （UA） and asiatic acid （AA） on Ca2＋ -induced liver mitochondrial permeability transition （MPT）. Method： Effects of three compounds on liver MPT induced by Ca2＋ were assessed by measuring the change in mitochondrial swelling, mitochondrial membrane potential and release of matrix Ca2＋ in vitro. Result： Obvious mitochondrial swelling, loss of mitochondrial membrane potential and release of matrix Ca2＋ occurred after the addition of 50 μmol L- 1 Ca2＋. However, preincubation with 50 mg L- 1 OA, UA or AA significantly blocked the above changes. In addition, it was also found that there are differences in the inhibitions of three compounds on liver MPT induced by Ca2＋. Conclusion： Three pentacyclic triterpenoids, OA, UA and AA, have significant mitochondrial protection through blocking on liver MPT and the inhibition on liver MPT of AA is stronger than that of UA and OA.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第4期496-499,共4页
China Journal of Chinese Materia Medica
基金
江苏省高校自然科学研究计划项目(08KJB360011)
江苏省自然科学基金项目(BK2009172)
江苏省"333工程"
"青蓝工程"计划项目
江苏省滩涂生物资源与环境保护重点建设实验室项目(JLCBE10006)
关键词
齐墩果酸
乌苏酸
积雪草酸
肝细胞线粒体
线粒体高通透性转运
oleanolic acid
ursolic acid
asiatic acid
liver mitochondria
mitochondrial permeability transition
