摘要
目的：观察齐墩果酸（ＯＬＡ）对小鼠肝内谷丙转氨酶（ＡＬＴ）及谷草转氨酶（ＡＳＴ）的直接作用。方法：利用醋氨酚在整体小鼠肝脏及原代培养的小鼠肝细胞造成损害以取得ＡＬＴ及ＡＳＴ样品，随后用不同浓度的ＯＬＡ混悬液分别与ＡＬＴ及ＡＳＴ样品作用，并测定作用前后这两种酶活性的变化。结果：ＯＬＡ对ＡＬＴ具有直接的抑制作用，而对ＡＳＴ则没有类似作用。结论：本实验结果提示，在对ＯＬＡ的肝保护机制进行研究时以及临床应用ＯＬＡ治疗肝炎时，应慎用ＡＬＴ作为判定肝损害的指标。
OBJECTIVE: To observe the effects of oleanolic acid (OLA) on the activity of alanine
aminotransferase (ALT) and aspartate aminotransferase (AST) in mouse liver. METHODS:
Mouse liver damage and primarily cultured mouse hepatocyte injury were induced by
acetaminophen (AAP) to get the ALT and AST samples.Then OLA with different concentrations
reacted with the ALT and AST samples.The activity of ALT and AST were determined. RESULTS:
OLA directly inhibited the activity of ALT.Similar effect was not observed on AST. CONCLUSION
:The results suggested that when OLA will be used to treat hepatitis and OLAs mechanisms on
hepatoprotective effect will be studied,it will be cautious to adopt ALT as an index of hepatic
injury.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
1999年第6期377-379,共3页
Chinese Pharmaceutical Journal
基金
北京医科大学医学师资奖学金资助
关键词
齐墩果酸
谷丙转氨酶
谷草转氨酶
肝损伤
小鼠
oleanolic acid,Author alanine aminotransferase (ALT),aspartate
aminotransferase (AST),activity of ALT and AST
