摘要
目的:建立HPLC法测定珍菊降压片中绿原酸与氢氯噻嗪的含量。方法:采用反相高效液相色谱法,C18色谱柱,流动相为甲醇-0.1%磷酸溶液(20∶80),检测波长为327 nm,流速为1.0 mL.min-1,柱温40℃。结果:绿原酸进样量在0.049 6～0.496μg(r=0.999 5)、氢氯噻嗪进样量在1.002～10.02μg范围内(r=0.999 8)线性关系良好,该制剂中绿原酸9次测定的平均回收率为101.0%,RSD 2.0%;氢氯噻嗪9次测定平均回收率为100.1%,RSD 1.4%。结论:本方法简便、准确、可靠,可用于珍菊降压片中绿原酸与氢氯噻嗪的含量测定。
Objective： To establish an HPLC method for determination of chlorogenic acid and hydrochlorothiazide in Zhenjujiangyapian. Method：The HPLC method was carried on Cls column using methanol-O. 1% phosphoric acid （20： 80）as mobile phase, and the detection wavelength was 327 nm, the flow rate was 1.0 mL min - 1 and the temperature of column was 40 ℃. Result： In the HPLC method, the calibration curve for chlorogenic acid, hydroehlorothiazide were linear in the range of 0.049 6-0. 496 （ r = 0.999 5 ） and 1. 002-10.02 μg （ r = O. 999 8 ）. The average recovery for ehlorogenic acid, hydrochlorothiazide were 101.0% and 100. 1%. RSD were 2.0% and 1.4% （ n = 9 ）, respectively. Conclusion ： The method is convenient, precise and reliable for determining the content of chlorogenic acid and hydrochlorothiazide in Zhenjujiangyapian.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第4期481-483,共3页
China Journal of Chinese Materia Medica
