摘要
目的：观察重复缺氧对小鼠在体脑乳酸含量以及Ｈ２Ｏ２对荷兰猪离体突触体乳酸含量的影响，并分析其作用机制。方法：采用酶氧化方法对突触体培养液中乳酸含量进行测定。结果：单次急性缺氧鼠脑内乳酸含量高，但在重复缺氧的作用下，鼠脑内乳酸的含量并未随缺氧时间的延长而增加。在突触体培养液中分别加入Ｈ２Ｏ２、ＦＣＣＰ、Ｒｏｔｅｎｏｎ，乳酸浓度比正常对照组显著性的增高。对突触体进行长时间Ｈ２Ｏ２处理，乳酸含量未见显著升高或降低。同时在突触体培养液中加入Ｈ２Ｏ２和ＦＣＣＰ，乳酸浓度相当于单独在突触体培养液分别加入Ｈ２Ｏ２与ＦＣＣＰ产生乳酸之和。结论：重复缺氧与Ｈ２Ｏ２处理可能激发脑与突触体内的抗自由基损伤防御系统；突触体从有氧呼吸到无氧酵解的转换主要不是由于Ｈ２Ｏ２对突触体膜的损伤。
Aim: Effects of repeated exposure to hypoxia and H 2O 2 treatment on content of
lactate were observed in the brain of mice and the synaptosomes of guinea pigs. Methods:
Lacate production was detected by the enzymatic oxidation. Results: The content of lactate was
increased in group exposed to hypoxia for once whereas on further increase was seen in group
of repeated hypoxia exposure. Lactate concentration was increased when synaptosome culture
was treated respectively by H 2O 2, FCCP and rotenone. No significant change was shown
when the synaptosomes were exposed to H 2O 2 for longer period of time. The increase in
content of lactate in group exposed to H 2O 2 and FCCP simulataneously was almost equal to
the sum of changes in both group H 2O 2 and FCCP. Conclusion: Some protective systems of
antifree radicals are triggered by exposure to repetitive hypoxia and H 2O 2,and the transition
from respiration to glycolysis in the synptosomes seems not due mainly to damage to their
membrane.
出处
《中国应用生理学杂志》
CAS
CSCD
1999年第2期165-168,共4页
Chinese Journal of Applied Physiology
