摘要
采用光棘豆无菌苗胚轴诱导的分化能力强的愈伤组织建立的胚性细胞悬浮系材料，对悬浮细胞原生质体解离所需酶液、原生质体培养所需要的渗透压和激素组合进行了研究。发现较低的培养基渗透压（＜ ０．３５ｍｏｌ／Ｌ 葡萄糖） 和较高浓度的２ ，４－ Ｄ（＞ １ｍｇ／Ｌ）易于诱导细胞出芽分裂，导致细胞破碎和死亡，并引起培养细胞褐化。找出了适合光棘豆悬浮细胞原生质体培养的培养基渗透压和激素组合。
Cell-suspension cultures derived from the calli initiated from plumalar axis of Oxytropis leptophylla were used for studying the effects of different enzymes, phytohormones and osmotic pressure in medium (e.g. the content of glucose) on protoplast isolation and culture. The results showed that the protoplasts were easy to damage into small segments when osmotic pressure was out of balance for protoplats' protoplasm. It was also found that the low osmotic pressure acted similarly to 2,4-D at high concentration in inducing the cell budding division, which resulted in death of cells. The optimum osmotic pressure and phytohormones combination was determined.
出处
《植物研究》
CAS
CSCD
北大核心
1999年第3期313-317,共5页
Bulletin of Botanical Research
关键词
光棘豆
原生质体
激素
渗透压
悬浮细胞
Oxytropis leptophylla
Protoplast
Phytohormone
Osmotic pressure
Cell-suspension
