摘要
本文采用A和B两种基本培养基,对15属31个种的豆科植物进行组织培养并得到再生植株。同时,探讨了培养基成分(大量元素、微量元素、有机成分和激素)、光照、温度及种子萌发率对愈伤组织分化的影响。对于多数豆科植物来说,A和B两种培养基的大量元素,B5培养基的微量元素和有机成分,1500—2500 lx自然光为主的光照和18—27℃的变温条件,以及当年采集的豆科植物种子,比较适合于愈伤组织的分化。根据豆科植物愈伤组织分化时形态结构特征上的变化,可将其分为a、b、c、d 4种基本类型,同属植物的愈伤组织基本上属于同一类型,不同属植物的愈伤组织往往属于不同类型。
Plant regenerations were achieved from tissue cultures of 31 species of 15 genus of legume plants on A and B media.Various factors including medium composition (major elements, minor elements,organic components and phytohormones),seed germination rate,illumination and temperature conditions were tested for their effects on callus differentiation in tissue cultures. Combinations of major elements of A or B medium,minor elements and organic components of B5 medium,illumination of 1500-2500 1x,and temperature of 18-27℃ were found suitable for callus differentiation of most legume plants.The calli induced from freshly collected seeds had higher differentiation capacity than from seeds sored for three years.Four types(a,b,c and d) of callus were morphologically distinguished during the differentiation in legume plant tissue cultures.Generally,calli from plants of the same genus belonged to the same type.
关键词
豆科植物
组织培养
植株再生
Legume plants
Tissue cultures
Plant regenerations
