摘要
以芦荟叶片为试材,采用酶解方法进行原生质体分离,并在此基础上对斑纹芦荟和不夜城芦荟进行原生质体融合。结果表明,以芦荟的叶肉细胞为试材,可成功分离出原生质体,而且活力较高,达90%以上。聚乙二醇(PEG)融合的结果可获得部分融合体。芦荟原生质体的成功分离主要取决于材料的来源、酶液的组成,另外还受酶解液的渗透压、温度和pH值等影响。融合的效率主要受融合剂的浓度、原生质体的生理状态及处理的时间等影响。
The protoplast of Aloe vera L.was isolated from its leaves by enzymolysis method.Then the protoplast fusion of Aloe nobilis and Aloe vera L.var.chenensis was induced by PEG.As a result,the protoplast was successfully gotten from the mesophyll cells of Aloe vera L.with the higher activity over 90%.Some fusants were obtained by PEG induction successfully.Protoplast isolation of Aloe vera L.was mainly related with the plant material and the component of enzyme solution,and also affected by osmotic pressure,temperature and pH value.The fusion efficiency was affected by PEG concentration,physiological characteristics of protoplast and processing time.
出处
《山东农业科学》
2010年第10期27-29,共3页
Shandong Agricultural Sciences
基金
2009年河南省教育厅立项项目(编号:2009A180014)
关键词
芦荟
原生质体
分离
融合
Aloe vera L.
Protoplast
Isolation
Fusion
