摘要
对一株戈登氏菌产类胡萝卜素的发酵培养基进行了研究。采用单因素设计的方法筛选最适宜发酵产类胡萝卜素的氮源、碳源,采用Plackett-Burman设计筛选出对发酵产胡萝卜有重要影响的无机盐类。结果表明戈登氏菌发酵产类胡萝卜素最适碳源为蔗糖,最适氮源为KNO3。MgSO4,FeSO4这两种无机盐对菌株产类胡萝卜素具有显著性的影响。最终确定发酵培养基为:蔗糖20g/L,硝酸钾4g/L,MgSO4 0.4g/L,FeSO4 15mg/L,KH2PO4 2g/L,Na2HPO4 3g/L,柠檬酸钠2g/L。用此培养基,戈登氏菌产类胡萝卜的量可达到600.5mg/L,比最初所选用的培养基提高了212.5%。
Fermentation medium for carotenoids production by a gordonia strain were investigated. The single factor design method were used to screen the most suitable carbon source and nitrogen source for carotenoids production,Plackett- Burman design was adopted to screen inorganic salts effecting on carotenoids fermentation. The Results showed that most suitable carbon source and nitrogen source for carotenoids fermentation by gordonia was sucrose and KNO3, separately. MgSO4, FeSO4 showed significance influence on carotenoids fermentation. The fermentation medium were screened as followings, 20g/L sucrose, dg/L KNO3, 0.4g/L MgSO4, 15mg/L FeSO4, 2g/L KH2PO4, 3g/L NazHPO4, 2g/L sodium citrate. Using specific formation medium, The strain gordonia could produce 600.5mg/L carotenoid, increasing by 212.5% compared with the initial fermentation medium.
出处
《食品与发酵科技》
CAS
2011年第1期42-45,共4页
Food and Fermentation Science & Technology
基金
发酵工程省部共建教育部重点实验室开放基金(项目编号:2009KFJJ06)
