摘要
由甘薯叶片诱导出愈伤组织，并由此建立起悬浮培养细胞系．分析其生长表明：南薯８８和８１２９４两种细胞系生长曲线均呈“Ｓ”型，其中南薯８８生长较快，是８１２９４的３倍以上，１０ｄ培养物的鲜重是初始鲜重的１０倍．培养条件研究结果表明：南薯８８细胞悬浮培养的最佳２，４Ｄ质量浓度为１．０ｍｇＬ－１，最佳蔗糖质量浓度为３０ｇＬ－１，椰乳和酵母汁能明显促进其生长；对于８１２９４，２，４Ｄ最佳质量浓度为２．５ｍｇＬ－１、蔗糖最佳质量浓度１５ｇＬ－１，酵母汁可促进其生长，但椰乳却无效；谷氨酰胺、色氨酸和脯氨酸不能促进甘薯悬浮细胞的生长，水解酪蛋白对其生长的促进作用亦不大；
Cell suspension culture of two sweet potato( Ipomoea batatas L. Lam) genotypes, Nanshu 88 and 8129 4, was established from the calli derived from leaves. The growth curve of two clones of cell suspension was sigmoid, and the growth rate of Nanshu 88 was three times higher than that of 8129 4 on average. The cell fresh weight of 10 day culture of Nanshu 88 was 10 times higher than that of initiation. For Nanshu 88 optimal concentration of 2,4 dichlorophenoxyacetic acid (2,4 D) and sucrose in MS medium was 1.0 mg L -1 and 3 g L -1 respectively, and addition of coconut milk and yeast extract increased the growth. For 8129 4 the optimal concentration of 2,4 D and sucrose in the medium was 2.5 mg L -1 and 1.5 g L -1 respectively, and coconut milk had no effect on growth, but yeast extract stimulated growth dramatically. The casein hydrolysate had a little effect on the growth of the cell suspension culture, and single amino acids added into the medium (such as glutamine、tryptophan and proline) could not increase the growth at all. Glucose was shown to decrease the growth rate.
出处
《应用与环境生物学报》
CAS
CSCD
1999年第3期275-278,共4页
Chinese Journal of Applied and Environmental Biology
基金
四川省"九五"重点科学技术项目
关键词
甘薯
细胞悬浮培养
生长
培养基
Ipomoea batatas
cell suspension culture
growth
medium
