摘要
该文以转基因食品黑花生为研究对象，针对硒易挥发的特点，建立了高压密闭消解一石墨炉原子吸收光谱法测定黑花生中总硒含量的测定方法。研究表明，HNO3-H2O2可使样品达到最佳消解，以Pd（NO3）2＋Mg（NO3）2为基体改进剂，最佳灰化温度和原子化温度分别为500℃和2000℃．在优化实验条件下，该方法测定硒的线性范围为10～200μg·L^-1，检出限（3σ）为8．78μg·L^-1，空白样品的相对标准偏差（n=11）为4．6％，回收率为96％～113％。测定发现，黑花生果仁及包衣中均富含硒，且含量高于同一产地出产的另外两个品种。
The trace levels of selenium content in black peanuts were determined by graphite furnace atomic absorption spectrometry（GF-AAS）. The sample was ingested with high-pressure closed vessel using nitric acid - hydrogen peroxide as solvent. Pd （ NO3 ） 2 ＋ Mg （ NO3 ） 2 were selected as chemical modifiers to increase the thermostability of selenium, and the pyrolysis temperature and the atomization temperature for the experiments were seted at 500 ℃ and 2 000 ℃ , respectively. Under the optimal conditions, the calibration curve showed a good linear relationship between the adsorption peak area and selenium concentration in the range of 10 -200 μg· L^-1 with a detection limit（3σ） of 8.78 μg· L^- 1. The relative standard deviation （ n = 11 ） of blank sample was 4.6% , and the recoveries varied from 96% to 113% . The result also indicated that the kernels and coats of black peanuts contained trace selenium and their contents were higher than those of other two local peanuts.
出处
《分析测试学报》
CAS
CSCD
北大核心
2011年第2期218-221,共4页
Journal of Instrumental Analysis
基金
山西省高校科技开发项目(200713033)
运城学院院级科研基金资助项目(20060257)
关键词
石墨炉原子吸收法
黑花生
硒
graphite furnace atomic absorption spectrometry
black peanut
selenium
