摘要
目的 研究结肠癌中ｐ１６ 基因的失活方式．方法 采用Ｓｏｕｔｈｅｒｎ ｂｌｏｔ 法鉴定了结肠癌细胞株ＳＷ４８０ ，Ｃｏｌｏｎ２０５ ，ＨＲ８３４８ ，ＨＴ２９ ，Ｃｏｌｏ３２０ ，ＬＳＩＧＴ９６９５ 及１８ 例正常结肠粘膜组织中ｐ１６ 基因的甲基化情况．结果 在６ 株结肠癌细胞株中有５ 株（８３－３ ％ ） ｐ１６ 基因ＣｐＧ岛呈异常甲基化状态，与之相对，１８ 例正常结肠粘膜组织中仅５ 例（２７－８ ％ ） 表现为异常甲基化．结论 在结肠癌中ｐ１６ 基因因异常甲基化而失活，这为结肠癌的基因治疗提供了新策略。
AIM To study the inactivation mode of p16 gene in colon cancer. METHODS The methylation rate of p16 gene in colon cancer cell lines (Colo205 , HT29 , SW480 , HR8348 , Colo 320 , LSIGT9695) and 18 cases of normal colonic tissues were examined by using Southern blot method . RESULTS The aberrant methylation of 5○CpGisland of p16 gene was found to be positive in 5 of 6 (83-3 % ) colon cancer celllines.In contrast,only 5 of 18(27-8 % )normal colonic tissues showed aberrant methylation of the 5○CpG island of p16 gene . CONCLUSION The p16 gene in colon cancerisinactivated by aberrant methylation . This can provide a new tactics and a new targetfor gene therapy of colon cancer.
出处
《世界华人消化杂志》
CAS
1999年第8期676-678,共3页
World Chinese Journal of Digestology
