摘要
应用聚合酶链反应（PCR）──单链构型多态性（SSCP）结合银染法对14例大肠癌p53基因的第4、第5－6和第7外显子进行了点突变的研究，结果共检测出6例点突变，而且发现各外显子的突变频率存在差异。另外，利用购自ATCC的两个探针（p53cDNA探针和pYNZ22探针）对大肠癌中p53基因的杂合性丢失进行了研究，在14例大肠癌中共检出6例杂合性丢失。将点突变检测结果同杂合性丢失结果进行比较分析，并着重探讨了大肠癌中p53基因失活导致肿瘤的作用方式。
The exons 4-7 of p53 gcne were examined in 14 colorectal Cancer patients by using PCR SSCP-silver staining method. The results showed 6 cases of point mutation and the mutation frcquencies of exons were differcnt from each other. p53 cDNA and pYNZ22 VNTR were used as probes to examine LoH(Loss of hctcrozygosity) of 14 co1orcctal cancers. 6 cases with LOH were found. The results of present rcscarch suggest that the mutation and LOH of p53 gene are critical events in the progress and develop ment of Cancer. There werc different kinds of inactivation model of p53 gene in the process of develop ment of cancer and transformation of cells.
出处
《遗传》
CAS
CSCD
北大核心
1996年第4期26-28,共3页
Hereditas（Beijing)
基金
江苏省自然科学基金
