摘要
以聚酰胺-胺(PAMAM)树状大分子修饰的硅胶为载体,胰蛋白酶为模型,考察了PAMAM的代数和固定化条件对酶的固载量及活力的影响。实验结果表明:选用3.0代PAMAM树状大分子修饰硅胶为载体固定化胰蛋白酶,酶促反应最适pH值为9.0,最适温度为60℃,对酪蛋白表观米氏常数Km为7.76 mg/mL,固定化胰蛋白酶表现出了较好的热稳定性、储存稳定性以及重复使用性。
PAMAM dendrimer modified silica was prepared for trypsin immobilization.The generation of PAMAM and the immobilization conditions were investigated.The results showed that the 3.0 G PAMAM dendrimer modified silica would provide high immobilization yields and enzymatic activity and it was employed for trypsin immobilization.The optimum temperature and pH of the immobilized enzyme were 60℃ and 9.0,respectively.The Km value for casein was 7.76 mg/mL.Compared with free enzyme,the immobilized enzyme has better thermo-stability,and it also features good storage stability and reusability.
出处
《分析科学学报》
CAS
CSCD
北大核心
2011年第1期6-10,共5页
Journal of Analytical Science
基金
国家自然科学基金(No.90713013)
