摘要
目的观察虫草素体外对急性早幼粒细胞性白血病细胞(NB4细胞)的抑制增殖和诱导凋亡作用。方法将0.5,1.0,1.5,2.0 mg.mL-1虫草素在体外与NB4细胞共同培养,采用细胞计数、噻唑蓝(MTT)法检测不同浓度虫草素在24,48,72 h对NB4细胞增殖的抑制作用;用细胞瑞氏染色法、Hoechst33258荧光染色法、DNA亚二倍体检测法和线粒体膜蛋白检测法观察虫草素诱导NB4细胞凋亡的作用。结果与对照组比较,不同浓度虫草素均能显著抑制NB4细胞增殖,并呈剂量和时间依赖的量效关系;不同浓度虫草素作用24 h后均能显著促进NB4细胞凋亡。结论虫草素在体外能明显抑制NB4细胞增殖,并诱导细胞凋亡。
Objective To investigate the effect of cordycepin on NB4 leukemia cell proliferation and apoptosis in vitro. Methods NB4 cells were incubated with cordycepin（0.5,1.0,1.5,2.0 mg·mL-1）.The cells growth was tested by cell counting and MTT assay after co-culturing for 24,48,72 h.The apoptosis of NB4 cell was detected by cell morphology analysis,Hoechst 33258 fluorescence staining,DNA diploid content test and mitochondrial membrane protein assay. Results Different concentrations of cordycepin obviously inhibited the growth of NB4 cells in a time and dose dependent manner compared with the control.Apoptosis was significantly induced by different concentrations of cordycepin. Conclusion NB4 cells growth inhibition and apoptosis could obviously induced by cordycepin in vitro.
出处
《医药导报》
CAS
2011年第2期173-176,共4页
Herald of Medicine
关键词
虫草素
NB4细胞
增殖
细胞凋亡
Cordycepin
NB4 cell line
Proliferation
Apoptosis
