摘要
为进一步提高枯草芽孢杆菌(Bacillus subtilis)液态发酵产凝乳酶(milking-clotting enzyme,MCE)的能力,采用单因素试验和响应曲面法对枯草芽孢杆菌液态发酵的产酶条件进行研究和优化。结果表明:最优发酵工艺为:葡萄糖添加量16.2g/L,在500mL三角瓶中装53.3mL料液,接种量为体积分数0.130%,发酵时间120.43h,预测酶活力为1097.30SU/mL,经实验验证,在该条件下发酵产凝乳酶的酶活力为(1129.05±74.55)SU/mL,与模型预测值相符。
In order to improve the yield of milk-clotting enzyme （MCE）, response surface methodology （RSM） was used to optimize the fermentation process for the production of milk-clotting enzyme from Bacillus subtilis under liquid fermentation. The results showed that the optimal parameters for the fermenting process were as follows： glucose 16.2 g/L, 53.3 mL medium in a 500 mL Erlenmeyer flask, inoculums size 0.130%, fermenting time 120.43 h, and the predicted MCE yield was 1097.30 SU/mL. Under the optimal conditions the MCE activity was （1129.05 ± 74.55） SUhnL, indicating that the optimizing process is effective.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第3期156-160,共5页
Food Science
关键词
凝乳酶
枯草芽孢杆菌
液态发酵
响应曲面法
优化
milk-clotting enzyme
Bacillus subtilis
submerged fermentation
response surface methodology
optimization
