摘要
研究了培养条件对黑曲霉液体发酵制备β-葡萄糖苷酶的影响及β-葡萄糖苷酶的表观酶学性质。麸皮是黑曲霉β-葡萄糖苷酶的较适诱导物。黑曲霉NL02以40g／L麸皮为碳源，在250mL三角瓶中装液40mL，接种量8％，初始pH值5．5，30℃、170r／min下培养10d，β-葡萄糖苷酶活力为19．12IU／mL。β-葡萄糖苷酶最适温度为65℃，40℃时稳定性较好；最适pH值为4．8，在pH值3．0～5．0之间稳定性较好。
The effects of culture condition on β-glucosidase preparation by Aspergillus niger in submerged fermentation and the apparent enzymatic properties of β-glucosidase were investigated. Wheat bran was the optimal inducer of β-glucosidase among tested carbon sources. The optimal culture condition of β-glucosidase preparation by A. niger in submerged fermentation was 40 mL medium in 250 mL shake flask, the inoculation was 8 %, the initial pH value was 5.5, the culture temperature and rotation speed of Incubator Shaker were 30 ℃ and 170 r/min, respectively, and the highest β-glucosidase activity of 19.12 IU/mL was obtained for 10 days cultivation. The optimal temperature and pH value of β-glucosidase reaction was 65 ℃ and 4.8, respectively. The β-glucosidase was stable under 40 ℃ and in the environment pH value of 3.0-5. 0.
出处
《生物质化学工程》
CAS
2008年第5期5-8,共4页
Biomass Chemical Engineering
基金
国家自然科学基金(30471361)
江苏省高校自然科学重大基础研究项目(06KJA22015)
关键词
黑曲霉
Β-葡萄糖苷酶
麸皮
Aspergillius niger
β-glucosidase
wheat bran
