摘要
目的研究乙型肝炎病毒（ＨＢＶ）前Ｃ区变异的临床意义。方法应用错配ＰＣＲ限制片段长度多态性分析，检测ＨＢＶ前Ｃ区１８９６位突变。结果６２０例乙型肝炎患者ＨＢＶ前Ｃ区１８９６位变异２６４例，变异率４２．５８％。在急性肝炎、慢性轻度、慢性中度、慢性重度、重型肝炎、肝硬化中，变异率分别为１２．５％、３５．０％、５５．９４％、４５．２６％、５４．７２％、４８．７６％。年龄、性别与变异无关，病程＞５年、谷丙转氨酶（ＡＬＴ）异常、抗ＨＢｅ（＋）者变异率均明显增高。结论ＨＢＶ前Ｃ区变异与肝病严重性有一定关系。
Objective To investigate
HBV pre-C mutation rate and it's clinical significance . Methods HBV pre-C at 1896 mutation was
detected by mis-pair PCR restriction fragment length polymorphism(RFLP). Results 264 out of
620 patients with HBV infection had HBV pre-C mutation ,the total mutation rate was 42.58%.
Among acute hapatitis B,mildmiddle severe chronic hepatitis B and cirosis, the mutation rates
were 12.50%35.00% 55.94%45.26%54.72% and 48.76% respectively. It was obviously higher in
patients with disease durability over five years,or abnormal ALT and anti-HBe positive.
Conclusion HBV pre-C at 1896 mutation was relative with severity of liver diseases.
