摘要
目的研究重度子痫前期患者血清对NK细胞杀伤活性影响。方法重度子痫前期患者组(病例组)、正常妊娠妇女组(对照组)各15例,采用1∶1配比的病历对照研究。采用Percoll不连续密度梯度方法分离健康非孕妇女NK细胞,用10%、20%、40%浓度重度子痫前期患者血清及20%正常妊娠妇女血清孵育NK细胞20 h,。采用MTT法检测不同血清对NK细胞杀伤活性影响。结果在用20%重度子痫前期患者血清及正常妊娠血清孵育20 h后,重度子痫前期组NK细胞杀伤活性为(48.68±8.47)%,正常妊娠组为(35.21±8.24)%。与正常妊娠组相比,重度子痫前期组NK细胞杀伤活性增强,差异有统计学意义(P<0.01)。在重度子痫前期组,用10%浓度血清孵育20 h后,NK细胞杀伤活性为(39.98±7.65)%,40%浓度血清组为(58.33±7.45)%,重度子痫前期组中10%、20%、40%浓度血清组间两两相比,差异有统计学意义(F=24.326,P<0.01)。结论子痫前期患者血清中某些因子可使NK细胞杀伤活性增强。
Objective To study the effect of the sera from severe preeclamptic patients on killing ability of peripheral blood NK cells.Methods NK cells from healthy non-pregnant women were incubated with 10%,20% and 40% serum from severe preeclampsia patients and normal pregnant women for 20 hours.The effect of the sera on NK killing ability to K562 cells was analyzed by MTT method.Results In 20% severe preeclamptic sera group,NK killing ability to K562 cells [（48.68±8.47）%] was significantly increased compared with that in normal pregnant group [（35.21±8.24）%]（P 〈 0.01）.The killing ability of NK cells was（39.98±7.65）% in 10% severe preeclamptic sera group,while（58.33±7.45）% in 40% severe preeclamptic sera group.There were significant differences among 10%,20% and 40% severe preeclamptic sera group（F =24.326,P 〈 0.01）.Conclusion The sera from severe preeclamptic patients might increase the killing ability of NK cells.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2011年第2期137-139,共3页
Journal of China Medical University
基金
辽宁省博士启动基金资助项目(20071054)
