摘要
目的研究子痫前期患者外周血单个核细胞分泌Th1/Th2细胞因子功能变化。方法子痫前期患者29例,正常妊娠妇女15例,分离外周血单个核细胞,体外培养72小时,ELISA方法检测培养上清液中Th1型细胞因子IL-2、IFN-γ及Th2型细胞因子IL-4、IL-10水平。结果子痫前期组PBMC培养上清液中,Th1型细胞因子IL-2、IFN-γ水平(259.4±90.5)pg/mL,(452.0±122.6)pg/mL与正常妊娠组(140.3±73.2)pg/mL,(307.5±106.4)pg/mL相比,显著升高,分别为P<0.01,P<0.05;Th2型细胞因子IL-4、IL-10水平(28.8±7.7)pg/mL,(178.9±98.6)pg/mL与正常妊娠组(41.9±11.4)pg/mL,(316.1±284.6)pg/mL相比,显著下降,分别为P<0.01,P<0.05。结论子痫前期患者外周血单个核细胞分泌Th1型细胞因子功能增强,分泌Th2型细胞因子功能减弱。
[Objective] To study the Th1/Th2 immunity in preeclampsia. [Methods] 29 patients with preeclampsia and 15 healthy pregnant women were selected by random, peripheral blood mononuclear ceils （PBMCs） were separated and cultured for 72 hours. Th1-type cytokines （IL-2 and IFN-γ） and Th2-type cytokines （IL-4 and IL-10） produced by PBMCs were detected by ELISA. [Results] In preeclamptic patients, concentrations of IL-2 （259.4±90.5） pg/mL in PBMC cultural supematants were significantly higher than in normal pregnant women （140.3± 73.2） pg/mL （P 〈0.01）. and IFN-γ also increased significantly （452.0±122.6） pg/mL versus （307.5±106.4） pg/mL, P 〈0.05）. Concentrations of IL-4 and IL-10were significantly decreased in preeclamptic patients than in normal pregnant women. （28.8±7.7） pg/mL versus （41.9±11.4） pg/mL, P 〈0.01, and （178.9±98.6） pg/mL versus （316.1±284.6） pg/ mL, P 〈0.05, respectively. [Conclusion] There is Th1/Th2 imbalance with increased Thl-type immunity and decreased Th2 -type immunity in preeclampsia.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第16期2414-2415,2425,共3页
China Journal of Modern Medicine
