摘要
结合工厂大豆分离蛋白(SPI)生产实际情况,研究了二次碱提过程中影响蛋白质提取率的主要因素。通过单因素实验和正交实验优化确定的最佳二次碱提工艺参数为:温度45℃,时间25min,料液比1:10,pH 8.40。将优化后的生产工艺与实验室新开发的工艺(三次碱提工艺)对高蛋白大豆粕蛋白质提取率的影响进行对比,结果表明新开发的工艺可有效降低高蛋白大豆粕残渣中蛋白质含量至20%以下。
According to the actual process of soy protein isolate （SPI） （ alkaline extraction for 2 times - acid precipitation） , the effects of different extracting conditions of the second alkaline extraction procedure on the yield of SPI were studied. Optimum conditions of the second alkaline extraction procedure were determined by single factor test and orthogonal test as follows ： temperature 45 ℃ , time 25 rain, ratio of solid to liquid 1： 10, pH 8.40. On the basis of the study, a new process of SPI （alkaline extraction for 3 times- acid precipitation） was developed, and the two processes were compared. The results showed that the new extraction process could effectively reduce the protein of the residue under 20% when high - protein soybean meal was used as a stuff.
出处
《中国油脂》
CAS
CSCD
北大核心
2011年第2期16-19,共4页
China Oils and Fats
关键词
高蛋白大豆粕
大豆分离白
提取工艺
high protein soybean meal
soybean protein isolate
extraction process
