摘要
目的:探讨SHH,Notch信号通路和其下游信号分子Gli-1、Hes-1在人子宫内膜癌的表达及意义。方法:应用免疫组织化学方法检测37例正常人子宫内膜、增生内膜和子宫内膜癌中SHH、Notch信号的表达,同时采用RT-PCR方法检测上述新鲜组织中Gli-1、Hes-1的表达。结果:子宫内膜增生组和子宫内膜癌组SHH、Notch的表达均强于正常组(P<0.05)。正常组和子宫内膜增生组Gli-1的表达类似,在子宫内膜癌组表达较低;正常组和子宫内膜增生组Hes-1的表达类似,在子宫内膜癌组表达升高。经随访,本研究选取的病例现均存活。结论:SHH和Notch表达与子宫内膜癌的发生密切相关,Gli-1和Hes-1主要参与子宫内膜癌的进展变化,但可能作用不同。
Objective：To explore the expression and significance of sonic hedgehog（SHH）,Notch and their effective signaling factor in endometrial carcinomas.Methods：The expression of SHH and Notch was examined in normal endometrial tissues,hyperplasia and carcinomatous endometrium by immunohistochemistry.Gli-1 and Hes-1 as effective factor of SHH and Notch signaling on the endometrial carcinoma were also examined by RT-PCR.Results：The expression of SHH and Notch was very weak in normal endometrium,but was increased in endometrial hyperplasia and carcinoma stepwisely with significant differences.There was no marked difference in the expression of these molecules in carcinomas according to stages and histologic grades.The expression of Hes-1 mRNAs was weak in normal endometrial tissues,with a slight predominance in the proliferative phase and endometrial carcinomas.Gli-1 mRNA was weak in endometrial carcinoma,the expression of Gli-1 mRNAs was increased in normal and hyperplasia endometrium.Conclusion：The abnormal activation of SHH and Notch pathway is involved in the endometrial carcinoma possibly in an auto/paracrine fashion,suggesting the possibility of the hedgehog and Notch pathway being a novel candidate for molecular targeting with different roles.
出处
《现代妇产科进展》
CSCD
北大核心
2011年第1期54-57,共4页
Progress in Obstetrics and Gynecology
