摘要
目的 探讨手术创伤对老龄大鼠海马环氧化酶-2（COX-2）和前列腺素E2（PGE2）的影响.方法 健康雄性SD大鼠45只,月龄18月,体重500～600 g,随机分为3组（n=15）：对照组（C组）腹腔注射生理盐水3 ml;麻醉组（A组）和手术组（S组）腹腔注射1%戊巴比妥钠50 mg/kg（稀释至3 ml）麻醉,S组麻醉下行腹腔探查＋阑尾切除术＋脾切除术.于麻醉或术后1、3、7 d（T1～3）时测定认知功能、海马COX-2 mRNA表达和PGE2含量.结果 与C组和A组比较,S组中央格时间延长,跨格及站立次数和正确反应次数减少,全天总反应时间延长,海马COX-2 mRNA表达上调,PGE2含量增加（P＜0.05）,C组和A组上述指标差异无统计学意义（P＞0.05）.结论手术创伤导致老龄大鼠术后认知功能障碍的机制可能与上调海马COX-2 mRNA表达和增加PGE2含量有关.
Objective To investigate the effect of surgical trauma on the expression of COX-2 and PGE2in the hippocampus in aged rats. Methods Forty-five 18-month-old male SD rats weighing 500-600 g were randomly divided into 3 groups （n = 15 each）： group Ⅰ control （group C）; group Ⅱ anesthesia （group A） and group Ⅲ surgery ＋ anesthesia （group S）. Anesthesia was induced by intraperitoneal 1% pentobarbital sodium 50 mg/kg in group A and S. The animals underwent appendectomy and splenectomy under anesthesia in group S.Cognitive function was assessed by open field test and Y-mase test on the 1st, 3rd and 7th day after anesthesia and surgery （T1-3）. The animals were sacrificed after behavior tests at T1.2.3 and the hippocampi were removed for determination of the expression of COX-2 mRNA （by RT-PCR） and PGE2 content （by ELISA）. Results The time the animal spent in the central square was significantly prolonged, the number of crossing grid and standing on the back legs and the number of right response were decreased, the total reaction time was prolonged and the COX-2mRNA expression at T1 and PGE2 content in the hippocampus were increased at T1,2 in group S as compared with group C and A. There was no significant difference in the variables mentioned above between group C and A. Conclusion Surgical trauma can induce early postoperative cognitive dysfunction through up-regulation of COX-2 mRNA expression and by increasing PGE2 content in the hippocampus in aged rats.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第10期1195-1197,共3页
Chinese Journal of Anesthesiology
基金
国家自然科学基金青年基金（30901393）
武汉大学中南医院青年基金
湖北省卫生厅资助项目（JX4823）
关键词
外科手术
环氧化酶2
地诺前列酮
海马
老年人
Surgical procedures, operative
Cyclooxygenase 2
Dinoprostone
Hippocampus
Aged
