摘要
运用半定量RT-PCR技术对候选功能基因在表达水平上检测不同交配组合胚胎早期发育过程中存在的差异,选取正常DDK品系小鼠和BALB/c品系小鼠,进行不同组合交配.选取8个功能基因EGF,EGF-R,TGF-α,Bcl-2,Mcl-1,C-myc,H-ras,LIF作为候选基因.结果表明,在2细胞期有EGF,Bcl-2,Mcl-1 3个基因进行表达,且3个基因表达差异显著(P<0.05);在4细胞期有6个基因进行表达,EGF,Bcl-2,Mcl-1,EGF-R4个基因表达差异显著(P<0.05);在8细胞期有7个基因进行表达,EGF,Bcl-2,Mcl-1,EGF-R4个基因表达差异显著(P<0.05).杂交胚胎从8细胞期之后即桑葚期开始发生凋亡现象,因胚泡形成障碍,而导致胚胎早期死亡;候选基因中Mcl-1,Bcl-2,EGF,EGF-R基因在胚胎细胞中表达量差异显著(P<0.05),并且与正常胚胎相比杂交胚胎中差异基因的表达均下调,这些基因表达水平的下调可能间接地引发早期胚胎致死现象.
This study using semi-quantitative RT-PCR at the expressing level detects the differential expression of candidate functional genes of different mating combinations during the early period of embryonic development.And select the normal DDK strains of mice and BALB/c strains to make different mating combinations of mice.Select 8 genes,namely,EGF,EGF-R,TGF-α,Bcl-2,Mcl-1,C-myc,H-ras,LIF as candidate genes.Results show that in 2-cell stage there are three genes EGF,Bcl-2,Mcl-1 expressing,and their expression is significantly different（P0.05）;At the 4-cell stage 6 genes express,the expression of 4 genes EGF,Bcl-2,Mcl-1,EGF-R being significantly different（P0.05）;At the 8-cell stage there are 7 genes expressing with the expression of 4 genes EGF,Bcl-2,Mcl-1,EGF-R being significantly different（P0.05）.In conclusion,hybrid embryos begin to apoptosis from the 8-cell stage,the stage of morula,due to the barrier of forming blastocyst,which leads to early embryonic death;The expression of candidate genes Mcl-1,Bcl-2,EGF,EGF-R in the three kinds of genetic background is significantly different（P0.05）,and it is small compared with normal hybrid embryos in embryonic gene expression differences.The lower level of gene expression may indirectly lead to early embryonic death phenomenon.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2010年第6期683-689,共7页
Journal of Henan Agricultural University
基金
河南省教育厅高校杰出科研人才创新工程项目(006KYCX015)
