摘要
利用ISSR分子标记技术,对湖南城步马尾松种子园的4个种源区的117个无性系的马尾松遗传多样性进行了研究。采用了10个ISSR引物扩增共获得了218个标记位点,其中有210个为多态位点,多态位点比率为96.33%。不同种源区多态位点百分率在88.99%~90.37%之间。其中两广种源区的多态位点百分率最高,达90.37%,说明其种源内遗传变异水平在4个种源区中是最高的。总的Shannon多样性指数为0.503 0。Nei’s遗传多样性指数为0.334 7。各个种源区的遗传一致度的变化范围在0.968 9~0.981 2之间,各群体间的基因分化系数为0.039 3,基因流为12.221 9。4个种源区间Nei’s遗传距离UPGMA聚类分析结果表明,遗传距离（0.019 0）最小的为雪峰山地种源区与贵州种源区首先聚类,雪峰山地种源区、贵州种源区与南岭山脉种源区依次聚类,遗传距离最大（0.031 6）的是两广种源区与贵州种源区,两广种源区在遗传距离上与其他3个种源区的聚类相距较远,这与其地理分布格局大致吻合。本研究的初步结果为无性系种子园的经营和择伐提供了一定的理论依据。
Genetic diversity of 117 clones in 4 populations of Pinus massoniana Lamb.seed orchard in Chengbu,Hunan Province was studied using ISSR markers.Ten ISSR primers,selected from 100 UBC primers,were applied to amplify all clones and a total of 218 markers were obtained.Among them,210 are polymorphic loci and the rate of polymorphic loci is 96.33%.The percentage of polymorphic loci between populations shared a range of 88.99%~90.37% and the highest（90.37%） was between Guangdong and Guangxi populations,which explained its ability adapts to circumstances or environment strongly and the hereditary change level is the highest among the populations.The Shannon diversity index and the Nei＇s diversity index were 0.503 0 and 0.334 7（why different？）.The percentage of genetic identity of the 4 populations ranged from 0.968 9 to 0.981 2 and the coefficient of genetic differentiation and gene flow were 0.039 3 and 12.221 9,respectively.The dendrogram based Nei＇s genetic distance shows that a high similarity was between Xuefeng and Guizhou populations with the distance of 0.019 0.But there was a lower similarity between Guizhou and Guangdong-Guangxi populations with the distance of 0.031 6.The results reveal their geographic origination,which Xuefeng,Nanling,Guizhou populations are adjacent to each others,while the genetic distance of Guangdong-Guangxi population was farther away from that of the above 3 populations.This result provides a scientific evidence for the management and choosing in Pinus massoniana Lamb.seed orchard.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2010年第12期6-10,共5页
Journal of Central South University of Forestry & Technology
基金
教育部博士点基金项目(20060538004)
"十一五"国家科技支撑计划课题(2006BAD24B0301)
湖南省教育厅重点项目(07A077)
湖南省教育厅优秀青年项目(09B112)资助
关键词
马尾松
种子园
遗传多样性
ISSR
Pinus massoniana Lamb.
seed orchard
genetic diversity
ISSR
