摘要
合成了雌二醇抗原,并获得了雌二醇单克隆抗体,通过酶联免疫吸附法测得效价为1.O×10~7.使用洗脱增强-时间分辨荧光免疫分析法测定了血清中的雌二醇,浓度范围为1.0×10^(-2)～1.0×10~3μg/L,最低检出限为5.6ng/L,批内RSD%小于3.7%,批间RSD%小于7.5%.将测定结果与放射免疫分析法作了比较.
A microtitre plate time-resolved fluorescence immunoassay (TRF1A) for the analysis of serum estradiol is described, involving competition between sample estradiol and an immobilized estradiol-bovine serum albumin sample for a monoclonal anti-estradiol antibody. The assay based on the qualification of the europium chelator, (chlorosulfonyl) thenoyltrifluoroacetone( CTTA) in the presence of excess Eu3+ insolution. The assay dose-response curve covered a ranger of 1×10-5～1×10-12 g/mL. The intra-andinter-assay coefficient of variation for the assay ranged from 0.6% to 3.7 % and from 1.9% to 7.5% respectively. The assay showed satisfactory correlation with a standard estradiol radioimmunoassay.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1999年第8期899-903,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助课题(29475186)
关键词
雌二醇
测定
时间分辨
荧光免疫分析
Estradiol, monoclonal antibody, time-resolved fluorescence immunoassay
