摘要
采用时间分辨荧光免疫分析(TRFIA)技术建立快速、高灵敏度的盐酸克伦特罗(CLB)全自动检测方法。以CLB-OVA包被96微孔板作为固相抗原,与游离CLB竞争限量的以Eu3+标记的抗CLB单克隆抗体,建立解离增强模式的荧光免疫分析体系检测CLB。该方法的灵敏度为0.03ng/mL,批内和批间变异系数分别为2.0%和8.9%,平均回收率为105.8%,与沙丁胺醇、莱克多巴胺的交叉反应率分别为0.96%和0.77%,结果表明,用TRFIA法检测CLB,灵敏度高、特异性强、稳定性好,具有良好的应用前景。
In order to provide a rapid, selectivity and very high sensitivity method for the determination of clenbuterel hydrochloride （CLB）, an indirect competitive time-resolved fluoroimmunoassay （TRFIA） was used. CLB-OVA was coated in a 96-well micro plate, anti-CLB monoclonalantibody-Eu^3＋ was added and incubated with standard CLB. suitability of the assay for quantification of CLB was also studied. Results showed the CLB detection limit was 0.03 ng/mL in the indirective TRFIA format. The within-run and between-run CVs were 2.0% and 8.9%. The recoveries from CLB-free urine which CLB standard was added were 105.8%. The cress reaction with salbutamol and ractopamine were 0.96% and 0.77%. The result showed that the TRFIA for CLB was high sensitivity, specificity and good stability, and it could be use in preparation of clenbuterol TRFIA kit.
出处
《中国食品学报》
EI
CAS
CSCD
2006年第1期6-10,共5页
Journal of Chinese Institute Of Food Science and Technology
基金
广州市科技攻关重点项目(No.2004Z2-E0031)
关键词
盐酸克伦特罗
时间分辨荧光免疫分析法
Clenbuterol Hydrochloride （CLB） Time-resolved fluoroimmunoassay （TRFIA）
