摘要
在ｐＨ７．２０－７．６０及离子强度低于０．０１２的介质中，耐尔蓝硫酸盐在核酸分子表面进行长距组装后，产生２９３．４ｎｍ，３４９．４ｎｍ和５６０．４ｎｍ的共振光散射（ＲＬＳ）增强峰。根据ＲＬＳ增强的Ｓｃａｔｃｈａｒｄ数据分析表明，ＮＢＳ在小牛胸腺ＤＮＡ，鱼精ＤＮＡ和酵母ＲＮＡ上的组装数分别是６．４，６．６和３．９，组装常数分别为７．１×１０＾６ｍｏｌ／Ｌ，４．６×１０＾６ｍｏｌ／Ｌ和１．７×１。
In the medium of pH ranging 7.20-7.60 and ionic strength lowering than 0.012, long range assembly of nile blue sulphate(NBS) on the molecular surfaces of nucleic acids occurs, resulting in three enhanced resonance light-scattering (RLS) peaks at 293.4 run, 349.4 run and 560.4 nm. Scatchard analysis by using the RLS enhancement data yields the assembly numbers and assembly constants, whichare 6. 4 and 7. 13×106mol/L for the assembly of NBS on the molecular surface of calf thymusdeoxyribonucleic acid(DNA), 6.6 and 4.58×106 mol/L for that of fish sperm DNA, 3.9 and 1.67×106 mol/L for that of yeast ribonucleic acid(RNA). By using the sum of the enhanced RLS intensity atthe three peaks, 0-0.8 mg/L calf thymus DNA and fish sperm DNA, 0.24～0.60 mg/L yeast RNA canbe determined with the determination limits of 0.4, 1.2 and 10.5 mg/L, respectively. The presentmethod was successfully applied to the determination of nucleic acids in synthetic samples.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1999年第11期1241-1247,共7页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(29875019)
重庆市中青年专家基金(97-4729)资助项目
关键词
耐尔蓝硫酸盐
核酸
长距组装
共振光散射
测定
Nile blue sulphate
nucleic acids
long range assembly
resonance light-scattering technique
