摘要
在低离子强度下，ｍｅｓｏ［四（对三甲铵基）苯基］卟啉（ＴＡＰＰ）能在核酸分子表面进行长距组装并诱导核酸超螺旋结构的形成．长距组装使得ＴＡＰＰ的Ｓｏｒｅｔ带发生减色效应，并使Ｓｏｒｅｔ带处激发产生的荧光被猝灭．体系离子强度（Ｉ）增大将降低ＴＡＰＰ长距组装的能力，导致荧光猝灭程度降低．在Ｉ＝０００４时，荧光猝灭程度与核酸的浓度成线性关系，可用于１５×１０－６～９０×１０－６ｍｏｌ／Ｌ的小牛胸腺ＤＮＡ，１５×１０－６～１０５×１０－６ｍｏｌ／Ｌ鱼精子ＤＮＡ和酵母ＲＮＡ的测定．
At low ionic strength,meso tetrakis[4 (trimethyammonium)phenyl]porphine(TAPP) can induce the formation of suprahelical helixes of nucleic acids because of its long range assembly on the surface of nucleic acid molecules,which leads to the hypochromic effect of the Soret band of TAPP and quenching of the fluorescence excited at the Soret maxima.The fluorescence quenching effect was decreased with of increasing ionic strength of the system.At ionic strength 0 004,linear fluorescence quenching was found in the range 1 5×10 -6 ～9 0×10 -6 mol/L of calf thymus DNA,1 5×10 -6 ～10 0×10 -6 mol/L of fish sperm DNA and yeast RNA.The determination results for 6 synthetic samples were satisfactory.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
1997年第6期648-653,共6页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家自然科学基金
重庆市中青年科技专家基金
西南师范大学自然科学基金
关键词
核酸
荧光猝灭
卟啉
TAPP
长距组装
超螺旋结构
Nucleic acids
meso tetrakis[4 (trimethyammonium)pheny]porphine(TAPP)
long range assembly
suprahelical helixes
fluorescence quenching
