摘要
本实验研究了ABA对花生侧根发生的影响。结果表明:用10μmol·L-1ABA浸泡处理花生种子1h或在含ABA的培养基上培养,均抑制侧根的发生,侧根发生率降低,数目减少,长度降低,发生的时间推迟1～2d;用ABA合成抑制剂25μmol·L-1NAPR浸泡后的种子,无论在1/2MS还是在含NAA培养基上培养,侧根发生率、侧根的数目和长度均增加。用NAA的极性运输抑制剂10μmol·L-1TIBA浸泡处理种子后,再在含ABA培养基上培养,侧根不发生,说明ABA抑制花生侧根的发生与种子内源ABA和IAA的水平相关。
In this study, the effect of ABA on the lateral root （LR） formation in peanut has been investigated. ABA treatment of seeds, either by soaking with 10 umol.L-1 ABA for 1 h or placed on the 1/2MS medium with ABA, could inhibit lateral root formation, with the decrease in LR formation ratio, in the LR number and in the LR length, and the delayed of LR formation time to 1-2 days. Seeds treated by soaking with 25 umol.L-1 naproxen, an inhibitor for ABA biosynthesis, and then grown on the 1/2MS medium or MS medium containing 5 umol.L-1 NAA had more LR formation percentage and LR numbers as well as LR lengths than that of the control seeds. No LR formation was observed, when seeds had been treated with 10 umol.L-1 TIBA, an IAA transport inhibitor, and then cultured on MS medium containing 10 umol.L-1 ABA for 8 days. Above results indicates that the inhibition of ABA on lateral root formation in peanut depends on the levels of endogenous ABA and IAA.
出处
《植物生理学报》
CAS
CSCD
北大核心
2011年第1期75-79,共5页
Plant Physiology Journal
基金
广东省自然科学基金项目(8151063101000011)
关键词
ABA
花生
侧根发生
ABA
peanut
lateral roots formation
