摘要
对丹参EST序列进行Blast分析,获得一个新的非特异性脂质转移蛋白基因,命名为SmLTP1(GenBank注册号为EF187461)。该基因cDNA全长593bp,包含一个长为357bp的开放读码框,编码118个氨基酸。生物信息学结构分析表明,该蛋白具有植物nsLTP的典型结构,即4对二硫键,4个α-螺旋,1个可结合和容纳脂肪酸分子的类似口袋状的疏水结构。实时荧光定量PCR分析结果表明,SmLTP1基因在丹参不同组织器官中差异表达,其表达受病原菌和茉莉酸甲酯的诱导,显示SmLTP1基因在植物防御反应中发挥作用。
By analyzing EST sequences of Salvia miltiorrhiza, we found a new gene which showed high homology with plant non-specific lipid transfer proteins and named as SmLTP1 （GenBank accession number： EF187461）. SmLTP1 consists of 593 nucleotides, including a single 357-bp opening reading frame and encoding a 118-amino-acid peptide. Bioinformatics analysis showed that SmLTP1 had the typical structure of the plant non-specific lipid transfer protein, including 4 pairs of disulfide, 4 or-helices and 1 tunnel-like hydrophobic cavity which could contain lipid molecules. Quantitative RT-PCR analysis revealed that the gene expressed in different organs, and could be induced by methyl jasmonate （MeJA） and pathogen. These results showed that the SrnLTP1 might be pathogen-responsive gene in plant defenses and involve in a complex gene regulation network.
出处
《植物生理学报》
CAS
CSCD
北大核心
2011年第1期63-68,共6页
Plant Physiology Journal
基金
国家自然科学基金(31000134)
陕西省科技研究发展(攻关)计划(2006K16-G15)
