摘要
以枸杞、新疆枸杞、宁夏枸杞的种子为外植体进行组培快繁技术的研究,结果表明,通过组培法获得的外植体污染率低,而水培法获得的外植体污染率高。枸杞最适宜的初代培养基为MS+6BA 1.5mg.L-1+IBA0.2 mg.L-1,宁夏枸杞和新疆枸杞最适宜的初代培养基为MS+6BA 2.5 mg.L-1+IBA0.2 mg.L-1;三种枸杞最佳的继代培养基均为MS+6BA 0.5 mg.L-1+IBA1.0 mg.L-1,分化诱导率分别为60%、40%、44%,增殖倍数分别为4.5倍、3.0倍、3.0倍;在MS+6BA 0.5 mg.L-1+IBA0.1 mg.L-1培养基上三种枸杞的幼苗生长均表现为粗壮;生根培养基以1/2MS+IBA0.1 mg.L-1效果最佳。以腐殖土1∶蛭石1∶河沙1等量混合配制的移栽基质效果最佳,移栽成活率为96%。
Seeds of Lycium chinense,Lycium barbarum and Lycium barbarum were cultured in tubes.The results were as follows： Contamination rate of explants from tissue culture was lower than those from water culture.the optimum primary culture medium for L.chinense was MS＋ 6BA 1.5 mg·L-1＋IBA0.2 mg·L-1,and MS＋ 6BA 2.5 mg·L-1＋IBA0.2 mg·L-1 was for L.barbarum and L.barbarum.The optimum successive transfer culture for all three species was MS＋6BA 0.5 mg ·L-1＋IBA1.0 mg·L-1,with which the rate of induction and differentiation were 60%,40%,and 44%,and the proliferation rate were 4.5,3.0 and 3.0 times,respectively.The seedlings of three species grew vigorously in medium of MS＋6BA 0.5 mg·L-1＋IBA0.1 mg·L-1.The optimum rooting medium was 1/2MS＋IBA0.1 mg·L-1.The best medium for transplantation found in this research was humus soil,vermiculite and river sand at 1∶1∶1,in which the survival rate of transplantation reached to 96%.
出处
《吉林林业科技》
2010年第5期8-11,36,共5页
Journal of Jilin Forestry Science and Technology
关键词
枸杞
组织培养
快繁技术
Lycium spp.； tissue culture； rapid propagation techniques；
