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基因重组鼠伤寒沙门氏菌防龋疫苗的研究Ⅰ.变形链球菌pac基因上游区序列测定和分析 被引量:1

Recombinant Salmonella typhimurium Anticaries Vaccine Ⅰ.Sequence of Streptococcus mutans Surface Protein pac Gene's Upstream
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摘要 目的 :对变形链球菌表面蛋白 pac基因上游区进行序列测定和分析。方法 :通过应用 PCR测序试剂盒和DNA自动测序仪 ABI3 10对变形链球菌表面蛋白 pac基因上游区进行序列测定。结果 :测定了 pac基因上游区内的5 17个碱基序列。结论 :变形链球菌表面蛋白 pac基因上游区序列测定为基因重组鼠伤寒沙门氏菌防龋疫苗的研究提供了基础资料。 Objective:To sequence the Streptococcus mutans surface protein pac gene's upstream. Methods: The PCR autosequence kit and autosequence instrument ABI310 were used for sequence. Results: The 517 base pairs sequence of the pac gene's upstream is sequenced. Conclusion: The sequence of pac gene's upstream will provide useful information to construct the recombinant Salmonella typhimurium anticaries vaccine.
作者 凌均棨 陈罕
出处 《华西口腔医学杂志》 CAS CSCD 北大核心 1999年第3期224-226,共3页 West China Journal of Stomatology
基金 广东省自然科学基金!(编号 970 10 5 )
关键词 变形链球菌 DNA pac基因 龋齿 防龋疫苗 Streptococcus mutans DNA sequence pac gene
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