摘要
目的 :构建携带变形链球菌表面蛋白pac基因A区片段的原核高效表达系统。方法 :对含变形链球菌表面蛋白pac基因的pPC41质粒进行PCR扩增 ,获得了pac基因A区片段 ;将扩增获得pac基因A区片段与表达质粒pET17 b定向克隆 ,重组质粒用BamHI,EcoRV双酶切后电泳鉴定。结果 :获得携带pac基因A区片段的重组质粒。结论
Objective:The objective of this study is to construct prokaryotic expressing plasmids carrying A region of S. mutans pac gene.Methods:The A region of pac gene was amplified by using PCR from plasmid pPC41 containing S.mutans surface protein pac gene. The new expression plasmid was constructed by using A region of pac gene and expressing plasmids pET17_b and directed cloning technique. The recombinant plasmid was identified by agarose gel electrophoresis after it was cut by using BamHI and EcoRV.Results:The expressing plasmid carrying pac gene A region was acquired.Conclusion:The recombinant plasmid provides useful experimental materials for further research work.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2001年第3期139-141,共3页
West China Journal of Stomatology
基金
广东省自然科学基金资助项目 (编号 970 10 5 )
