摘要
目的用携带ｃａｇＡ基因的Ｈｐ成功感染ＢＡＬＢ／ｃ裸鼠。方法分别采用ｃａｇＡ基因阳性和阴性的具有较强动力的新鲜Ｈｐ分离菌株，通过灌胃方式感染ＢＡＬＢ／ｃ裸鼠，感染后４周和８周分两批处死，进行微生物学和病理组织学检查。结果灌喂８周后，ｃａｇＡ＋实验组和ｃａｇＡ－实验组均成功定植Ｈｐ（１６／１６）；ｃａｇＡ＋组胃粘膜表现明显炎症及坏死病变，而ｃａｇＡ－组仅有轻微炎症发生。结论建立典型的Ｈｐ动物感染模型应选择ｃａｇＡ＋菌株。
Aim To infect successfully BALB/c nude mice with H.pylori carrying cagA gene. Methods Fresh clinical isolates of H.pyloriWTBZ with motive power were cultured in liquid media.40 BALB/c nude mice were randomized into cagA +5(16),cagA(16) and normal control groups.The nude mice of cagA+ group were orally inoculated broth culture of cagA + -Hp to stomach,and those of cagA- group were orally inoculated broth culture of cagA-- Hp. Results Hp colonized successfully the stomachs of all nude mice of cagA + and cagA + group. An obvious gastric pathology was observed in cagA + group,but only lower degree inflammation was observed in cagA-group. Conclusion Fresh clinical isolates of Hp with cagA gene and motive power can estabilsh a typical animal model of infection.
出处
《中国人兽共患病杂志》
CSCD
北大核心
1999年第4期6-8,共3页
Chinese Journal of Zoonoses
基金
国家重点科技攻关计划课题
