摘要
目的研究人胚胎中枢神经系统ＮＯＶｍＲＮＡ神经元的发育。方法地高辛标记的ｃＲＮＡ探针原位杂交技术。结果（１）脊髓：第１６周人胚胎脊髓腹侧出现ＮＯＶｍＲＮＡ神经元，为大型神经元，至第２８周扩展到后角中间神经元，第３８周中央灰质中阳性神经元明显。（２）延髓：第１６周延髓观察到下橄榄核、舌下神经核、迷走神经背核均有阳性神经元分布，至第２８周，三叉神经脊束核和楔束副核检测到阳性神经元；（３）脑桥、中脑和间脑：第３２周脑桥展神经核、中脑红核和黑质、丘脑腹外侧核和背内侧核均有阳性神经元分布。（４）大脑：第３２周纹状体有较多标记神经元，第３８周大脑皮质顶叶阳性神经元明显。以上结果表明ＮＯＶｍＲＮＡ神经元在人胚胎中枢神经系统中，首先在低级中枢出现，在高级中枢中逐渐发育；各胎龄标本中所观察到的阳性神经元多位于支配躯体运动的神经核团；随着胚胎中枢神经系统的发育，ＮＯＶｍＲＮＡ神经元逐渐增多，这与其他原癌基因在胚胎发育的早期表达高，而后降低不同。结论在人胚胎发育过程中ＮＯＶ基因对神经系统的分化和进一步的功能活动可能起重要调节作用，且这种作用逐渐增强。
Objective\ By using hIL 2 muscle model to exploit a new gene therapeutics for cancer.Methods\ Two mammalian expression vectors were constructed,which contained 1kb human interleukin 2 cDNA,and were transfected into primary skeletal muscle cells of the rats mediated by lipofectin(hIL 2 muscle model).The hIL 2 expression in primary muscle cells was detected by immunohistochemical stain method.Results\ Identification of pCMV IL 2 and PRSV IL 2 was confirmed by digesting with restrictive endonucleases,about 10% positive cells were observed and the expression level of pCMV IL 2 and pRSV IL 2 had no difference in the muscle cells.Conclusion Recombinant plasmid DNAs might be suitable for gene therapeutics for cancer.
出处
《解剖学报》
CAS
CSCD
北大核心
1999年第1期48-51,I013,共5页
Acta Anatomica Sinica
基金
95全军科研基金
