摘要
用地高辛标记的cRNA探针原位杂交方法研究了鲢、青蛙、蛇、鸡、牛、犬和猫脊髓中肾母细胞瘤过度表达基因（nov）mRNA阳性神经元的种系发育。结果显示，低等脊椎动物鲢、青蛙和蛇脊髓中仅有少量novmRNA阳性神经元，分布于灰质腹角。鸡脊髓中阳性神经元除主要分布于脊髓腹角外，中央灰质也有少量分布。哺乳动物牛、大和猫脊髓灰质中novmRNA阳性神经元分布广泛，背腹角、中央灰质及中央核区都检测到很强的杂交信号。以上结果表明，nov基因在从低等脊椎动物到高等脊椎动物的进化过程中非常保守，这种保守性提示nov基因在脊髓神经元发育、分化及正常生理功能中可能具有重要作用。
Systematic development of nephroblastoma overexpressed gene(nov) mRNA positive neurons was examined by in situ hybridization histochemistry using digoxigenin-labelled cRNA probes in spinal cord of silver carp,f rog,snake,chicken,cattle,dog and cat. Results were as follows. Nov mRNA positive neurons were only found in ventral horn of silver carp,frog and snake spinal cord- While,the positive neurons were aIso detected in central gray matter of chicken spinal cord besides ventral horn. Nov mRNA positive neurons were widely localized in the whole gray matter of cattle,dog and cat spinal cord,and the hybridization signal was very strong. The conservation of nov gene during evolution from lower vertebrate-fish to higher vertebratemammal supports the idea that it may play an important role in the development and difrerentiation of spinal cord neurons.
基金
95全军科研基金!96M096
关键词
肾母细胞瘤
过度表达
基因
脊髓
种系发育
nephroblastoma overexpressed gene (nov) mRNA in situ hybridization histochemistry spinal cord systematic development
