摘要
用紫外线和钴60放射线照射对产葡萄糖氧化酶的黑曲霉菌株P-9进行诱变,以含有2-脱氧-D-葡萄糖的选择培养基进行筛选,共获得7个耐受菌株,其中产酶活性最大的U-69菌株的葡萄糖氧化酶活力是出发菌株P-9的2·5倍。研究了U-69菌株合成葡萄糖酸钙的发酵条件,结果显示,最佳发酵条件为葡萄糖150g/L,碳酸钙400g/L,硫酸铵3～4g/L,pH5·5,250mL三角瓶装30mL发酵液,接种量10%,30～34℃发酵18h。
The glucose oxidase production capacity of Aspergillus niger P-9 was improved by mutagenesis using UV-or 60Co-irradiation and selected in 2-deoxy-D-glucose agar.7 strains which were resistant to 2-deoxy-D-glucose,4 strains had higher glucose oxidase production capacity than wild-type strain P-9.The glucose oxidase activity of the best producer,strain U-69（28.7U/mL）,was 2.5 times higher than strain P-9.The fermentation conditions of U-69 for calcium gluconate production were studied.The optimum fermentation conditions of U-69 to produce glucose oxidase were 150g/L glucose,400g/L CaCO4,34g/L（NH4）2SO4,30mL broth bottled in 250mL triangular flask,pH 5.5,inoculum size 10%,3034℃ and 18h.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第12期218-220,共3页
Science and Technology of Food Industry
关键词
黑曲霉
葡萄糖氧化酶
诱变
葡萄糖酸钙
Aspergillus niger
glucose oxidase
mutagenesis
calcium gluconate
